Sri Rahayu Lestari, Muhaimin Rifa'I
This study aims to explore the alternation expression of NFkB, activation degree of naïve T cells, and role of regulatory T cell. Hypercholesterolemia mouse model was created by giving a high-fat diet (HFD) for 45 days, and then they were treated by 12.5 mg/kg BW, 25 mg/kg BW and 50 mg/kg BW SGO for 30 days. The end of the treatment, the mouse was sacrificed, and the spleen was isolated to analyze cell surface molecules and regulatory T cells using flow cytometry. The data were analyzed by Cell Quest software and tested by statistical analysis One Way ANOVA Analysis. The results showed that a high-fat diet decreased the number of regulatory T cell and also decreased the expression of NFkB on CD4+ T cell in hypercholesterolemic mice. Moreover, the SGO did not show a significant effect on increasing the number of naïve T cells expressing CD62L molecule. Thus, the administration of SGO might inhibit activation of the NFkB pathway and decrease the stimulation of effector cell development that plays a role in the inflammatory pathway. © Published under licence by IOP Publishing Ltd.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang 5, Malang, 65145, Indonesia; Department of Biology, Faculty of Sciences, Brawijaya University, Jl. Veteran 42-44, Malang, East Java, 65145, Indonesia
