Melati Putri Pertiwi, Patricia Karin Himawan Praseptin, Ika Diana Werdani, Dwi Listyorini, Sitoresmi Prabaningtyas
Bioluminescent bacteria are a light-emitting organism. These set of species are found as free-living organism or symbionts which diversely spread in the terrestrial environment, freshwater and marine ecosystem. In Indonesia, a blue light emitting bioluminescent bacteria Photobacterium phosporeum was found in squid's ink sac, while the green light emitting one had not been identified. This study aimed to identify the species of bioluminescent bacteria emitting green light based on 16S rRNA gene. This study was conducted by isolating the total DNA of bioluminescent bacteria, then amplify 16S rRNA gene using a universal primer for bacteria. The sequence of amplified 16S rRNA gene then analyzed using Neighbour-Joining and Maximum Evolution methods in MEGA7 software and completed with intraspecific genetic distance analyses. The results show that bioluminescent bacteria emitting green light is a complex species with Photobacterium leiognathi or Photobacterium mandapamensis. © 2018 Author(s).
Department of Biology, Faculty of Mathematics and Natural Sciences, State University of Malang, Malang, Indonesia
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