Aloysius Duran Corebima, Mariana Rengkuan, Harissudin Masrur, Jahidin, Mohamad Nur Ibrahim
Tarsius spreading over Sulawesi mainland and on the surrounding islands including Buton Island is still known as an interesting animal to be studied, especially related to gene mutation. The objective of this descriptive explorative research is to analyze mutations at partial sequence of subunit II (COX2) cyt. oxidase gene of Tarsius tarsier form Buton. The sequencing of PCR product produced a base sequence of 560 nt. The gene sequencing results were then aligned with the genes of Tarsius dentatus and Carlito syrichta taken from Genebank (C. syrichta is assumed as the Sulawesian tarsier ancestor). It can be seen that the total number of the invariable sites and variable sites were 432 and 128 respectively, but the total number of mutation was 130. Subtitution mutations were found in 128 sites; the total numbers of transition and transversion mutations were 89 and 16 respectively without calculating the double and single subtitution at the same site of transition. Most of subtitution mutations in COX2 gene sequences occur in the third base of each codon. © 2019 Malaysian Society for Biochemistry and Molecular Biology. All rights reserved.
Universitas Negeri Malang, Malang, Indonesia; Universitas Negeri Manado, Tondano, Indonesia; Universitas Halu Oleo, Kendari, Indonesia
