Sofia Ery Rahayu, Amin Setyo Leksono, Zulfaidah Penata Gama, Hagus Tarno
Thailand and Indonesian papaya cultivars are two of the many cultivated papaya cultivars in East Java. In addition to fruit, papaya leaves are also widely used as traditional insecticides. The purpose of the study was to determine the active compounds, total phenols, total flavonoid, and antifeedant activity against Spodoptera litura F. larvae. Papaya Leaves were macerated with methanol. Phytochemical of leaf extract determined using LCMS. Total phenol and total flavonoids were measured based on the spectrophotometric method. The antifeedant activity was tested on the instar 3 phase larvae with the choice method. Feed leaves sprayed with different concentrations of papaya leaves extract and repeated ten times. The screening result showed that there are 62 compounds detected in Indonesian papaya extract and 60 compounds in Thailand extract. The highest total phenol content was found in Indonesian papaya extract. Meanwhile, the highest total flavonoid content was detected in Thailand extract. The treatment of 40% and 50% of both extracts significantly inhibited eating activity to 100%. Statistically, the combination of the type of extract and concentration showed significant antifeedant activity. According to the result, the difference in the antifeedant activity of extract caused by the difference of the phytochemicals content. The extracts of Thailand and Indonesian papaya leaf had the potential to be antifeedant for S. litura larvae. © 2020 American Institute of Physics Inc.. All rights reserved.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang No. 5, Malang, 65145, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya.University, Indonesia; Department of Pest and Disease, Faculty of Agriculture, Brawijaya University, Indonesia
