Fauzi Akhbar Anugrah, Rahmi Masita, Hanumi Oktyani Rusdi, Satrio Anggoro Putra
Medicinal plants cinchona (Cinchona Ledgeriana Moens.) is once an Indonesia's export commodity since many years ago. Unfortunately, the decline of cinchona production is currently also affected by plant-damaging fungal (Phytophthora sp.). This study aims to obtain endophytic bacteria in healthy cinchona plants. A number of 64 endophytic bacterial isolates evaluate for their ability to inhibit Phytophthora mycelia growth by dual culture technique. Nine isolates had inhibitory activity against fungal mycelium growth above 50% compared to control. The highest percentage of inhibition is shown by isolate B13 for about 84.31%. Many bacteria are motile and make calculations biased. Furthermore, an antagonistic confirmation test has carried out using a glass ring. Six of them still proved to be consistent with the ability to inhibit the mycelium growth of Phytophthora sp. Respectively, the inhibition percentage for isolate B13 was 68.32% ± 3.34, isolate D5 67.67% ± 0.92, isolate D13 were 65.43% ± 1.49, isolate B15 were 61.98% ± 1.18, isolate D11 were 58.91% ± 2.06, isolate D7 63.86% ±2.26, isolate A4 66.88% ±2.27, B14 isolates at 57.76% ± 2.84 and isolate A3 52.45% ±0.98. The novelty of this result becomes literature in the study of the potential of cinchona plant endophytic bacteria, which in the future can develop for bio-control applications that are safe for plants and the environment. © 2020 American Institute of Physics Inc.. All rights reserved.
Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Indonesia; Chemistry Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Indonesia
