I. Kade Karisma Gita Ardana, Zefry Okta Wardana, Vina Rizkiana, Nursasi Handayani, Hendra Susanto, Justinus Dwi Pratjojo Wisnubroto, Alfiyannul Akhsan, Vidi Vianney Chrisana Magrit Tanggo, Dwi Listyorini
East, Central, and West Java Provinces have the largest estimated number of people with breast cancer in Indonesia. Breast cancer can be caused by mutations in the RAD51 domain of BRCA2 genes. The purpose of this study was to determine the mutations and binding affinity of RAD51 domains of the BRCA2 proteins. This research also pursued to measure the expression of BRCA2 genes based on TNM stages of breast cancer. Total RNA was isolated from 22 samples provided by Saiful Anwar Regional Public Hospital, East Java, Indonesia. Mutation analysis conducted using Sanger sequencing. Gene expression analyzed using relative q-PCR with standardized OneWay ANOVA. The results of this study indicate that the expression of the BRCA2 gene did not increase significantly. Mutation analysis showed that there was a mutation in the RAD51 domain of the BRCA2 gene in the samples 17 and 19. Mutations that occur did not affect the binding domain structure area which in turn did not affect the binding affinity between the BRCA2 and RAD51 proteins. Moreover, there were no significant changes throughout the protein structure which in turn did not affect the binding structure. © 2020 American Institute of Physics Inc.. All rights reserved.
Department of Biology, Faculty of Mathematic and Natural Sciences, Universitas Negeri Malang, Jl. Semarang No. 5, Malang, 65145, Indonesia; Department of Oncology, Saiful Anwar Regional Public Hospital, Jl. Jaksa Agung Suprapto No. 2, Malang, 65112, Indonesia; Biotechnology Division, Central Laboratory of Mineral and Advanced Material, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang No. 5, Malang, 65145, Indonesia; PT. GeneCraft Labs Jakarta, Kebon Jeruk Business Park F2-9, Jl. Meruya Ilir No. 88, Meruya Utara, Jakarta, 11620, Indonesia
