Nina Bunga Anggraini, Annisa Sholihah, Elhah Nailul Khasna, Riri Wiyanti Retnaningtyas, Suharti, Dwi Listyorini
Indonesia home of abundant indigenous and hybrid rice varieties. The phenomenon of phenotipic-plasticity allows varieties or even the same species to exhibit different phenotypic characteristics when planted in different locations. Thus, the identification based on phenotypic characters is needed to be supported by genetic data. The aim of this research was to unveil the relationship between local rice varieties provided from East Java, Banten, and Samarinda based on matK and rbcL genes. Total DNA from leaves were used as template for amplification of both genes. Phylogenetic trees were reconstructed using Neighbour-Joining (NJ) models in Kimura-2 parameters with 1000 bootstraps. The phylogenetic analysis using matK proved that rice varieties from East Java were closely related to rice varieties from Banten rather than to rice varieties from Samarinda. Meanwhile the same study using the rbcL gave inconsistent results © 2020 American Institute of Physics Inc.. All rights reserved.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl Semarang, Malang, 65145, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl Semarang, Malang, 65145, Indonesia; Faculty of Biology, Universitas Gadjah Mada, Bulaksumur, Yogyakarta, 55281, Indonesia; Cell and Molecular Biology Program, Department of Biological Sciences, University of Arkansas, Fayetteville, 72701, AR, United States; Biotechnology Division, Central Laboratory of Mineral and advanced Material, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang Jl Semarang, Malang, 65145, Indonesia; PUIPT Disruptive Learning Innovation, Universitas Negeri Malang, Jl Semarang, Malang, 65145, Indonesia
