Dian Handayani, Muh A. Artasasta, Dian Mutia, Nurul Atikah, Rustini, Trina E. Tallei
Secondary metabolites from marine sponge-derived fungi repeatedly showed potential bioactive activity against several diseases. In this study, screening for antibacterial and cytotoxic isolated fungi from marine sponge Callyspongia sp. have been performed. A common media for culturing fungi, sodium dextrose agar (SDA), was used. For fungal isolates the streak plate method was used. Each pure fungal isolate was cultivated on rice media under room temperature for 4-8 weeks then extracted with ethyl acetate. Thirteen pure fungal isolates were successfully obtained from Callyspongia sp. Ethyl acetate extract of all isolated fungi was tested for antimicrobial activity against pathogenic microbes using the disk diffusion method and cytotoxic activity using brine shrimp lethality test (BSLT). Of thirteen fungal isolates, only Cas 02 showed antimicrobial activity against Staphylococcus aureus (SA) ATCC 2592 and Escherichia coli (EC) ATCC 25922 with a diameter of inhibition zone of 12.75 mm and 17.16 mm respectively. The cytotoxic activity results showed that only isolate Cas03 had a potential activity with LC50 <30 µg mL-1 and four fungal strains (Cas02, Cas06, Cas07, and Cas09) had a moderate activity with LC50 < 100 µg mL-1. Molecular identification showed that Cas02 was Aspergillus unguis, Cas03 was Penicillium citrinum, Cas06 and Cas07 were A. flavus, and Cas09 was A. austroafricanus. © 2021, BIOFLUX SRL. All rights reserved.
Sumatran Biota Laboratory, Faculty of Pharmacy, University of Andalas, Padang, West Sumatera, Indonesia; Biotechnology Department, Faculty of Mathematics and Natural Sciences, Malang State University (UM), Malang, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado, Indonesia
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