Elly Purwanti, Mohamad Amin, Siti Zubaidah, Maftuchah Maftuchah, Nur Hidayati, Ahmad Fauzi
Phaseolus lunatus L. is one of the legume plants found in some parts of Indonesia and has potential as alternative food rich in protein. This current research aimed at analysing genetic accessions of P. lunatus distributed in some areas in East Java, Indonesia, based on the RAPD (Random Amplified Polymorphic Deoxyribonucleic acid) marker. 15 accessions originated from four locations were analysed. Ten primers were used and produced 68 bands out of 67 were polymorphic. The percent polymorphism was 96% to 100%. Ten unique bands were detected in eight accessions (Prb1, Prb2, Prb5, Mdr12, Mdr16, Mdr19, Mdr4, and Mdr6). Using the Neighbor-Joining method, a phylogenetic tree was yielded by a similarity coefficient of 64% to 100%. On the genetic similarity coefficient (GSC) of 0.6, there were two clusters: the first and second major clusters (Cluster A and B). The former contained the accessions 7, 8, 13, and 14, while the latter comprised 2, 4, 16, 18, Prb1, Prb2, Prb3, Prb4, and Prb5. In conclusion, based on phylogenetic trees formed, P. lunatus from the same region cluster in the same cluster. © 2021 Jordan Journal of Biological Sciences. All rights reserved - Volume 14, Number 2
Department of Biology Education, Faculty of Teacher Training and Education, University of Muhammadiyah Malang, Jl. Raya Tlogmoas No. 246, Malang, 65144, Indonesia; Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Malang, Semarang No. 5, Malang, 65145, Indonesia; Department of Agrotechnology, Faculty of Agriculture and Animal Science, University of Muhammadiyah Malang, Indonesia; Department of Biology, Middle Tennessee State University, Murfreesboro, 37132, TN, United States