Muh. Ade Artasasta, Yanwirasti Yanwirasti, Muhammad Taher, Akmal Djamaan, Ni Putu Ariantari, Ru Angelie Edrada-Ebel, Dian Handayani
Sponge-derived fungi have recently attracted attention as an important source of interesting bioactive compounds. Aspergillus nomius NC06 was isolated from the marine sponge Neopetrosia chaliniformis. This fungus was cultured on rice medium and yielded four compounds including three new oxisterigmatocystins, namely, J, K, and L (1, 2, and 3), and one known compound, aspergillicin A (4). Structures of the compounds were elucidated by 1D and 2D NMR spectroscopy and by high-resolution mass spectrometry. The isolated compounds were tested for cytotoxic activity against HT 29 colon cancer cells, where compounds 1, 2, and 4 exhibited IC50 values of 6.28, 15.14, and 1.63 µM, respectively. Under the fluorescence microscope by using a double staining method, HT 29 cells were observed to be viable, apoptotic, and necrotic after treatment with the cytotoxic compounds 1, 2, and 4. The result shows that compounds 1 and 2 were able to induce apoptosis and cell death in HT 29 cells. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
Laboratory of Sumatran Biota, Faculty of Pharmacy, Andalas University, Padang, 25163, Indonesia; Biotechnology Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang (UM), Malang, 65145, Indonesia; Departement of Biomedical, Faculty of Medicine, Andalas University, Padang, 25163, Indonesia; Faculty of Pharmacy, International Islamic University Malaysia, Kuantan, 25200, Malaysia; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Udayana University, Bali, 80361, Indonesia; Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, The John Arbuthnott Building, 161 Cathedral Street, Glasgow, G4 0RE, United Kingdom
