S. Sugiharto, D. Merawati, H. Susanto, A. Pranoto, A. Taufiq
A passive lifestyle and lack of exercise are triggering factors in increasing metabolic syndrome, whereas metabolic syndrome is associated with increased morbidity and mortality. This study aimed to analyse an exercise-based nonpharmacological approach combined with instrumental music to the plasma levels of irisin. The study employed a randomised pretest-posttest-only group design recruiting 30 male participants. The individuals aged between 19-21 years, body mass index (BMI) 18.5-24.9 kg/m2, normal blood pressure, normal resting heart rate (RHR), VO2max 40-50 ml/kg bodyweight/min, normal haemoglobin (Hb). The subjects were randomly divided into three groups, MIE (n=10, moderate-intensity exercise), MIEM (n=10, moderate-intensity exercise + instrumental music), and HIE (n=10, high-intensity exercise). Blood sampling was collected from pre and 5 min post-exercise treatment. ELISA method was used to measure the circulating irisin levels. The non-parametric Kruskal-Wallis test, Mann Whitney test and Spearman correlation were chosen to analyse the data. The results showed that there is no significant difference in pre-exercise irisin levels (ng/ml) in MIE (1.58±0.89), MIEM (1.77±3.12), and HIE (1.65±0.88) with a P-value of 0.098. Importantly, the alteration of irisin levels post-exercise program revealed that the level of this peptide (ng/ml) is higher in HIE (7.28±5.11) compared to MIE (1.95±1.85) and MIEM (4.16±3.40) with a P-value of 0.019. The plasma levels of irisin significantly correlate to energy expenditure (r=0.353, P≤0.01), plasma peroxisome proliferator-activated receptor gamma coactivator-1α (r=0.597, P≤0.001), and high-density lipoprotein (r=0.513, P≤0.01). In summary, increased irisin secretion may directly contribute to the gradual alteration of energy expenditure and correlate to physical exercise intensity-related musical treatment. © 2021 Wageningen Academic Publishers
Department of Sport Science, Faculty of Sports Sciences, Universitas Negeri Malang, Malang, 65145, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang, 65145, Indonesia; Medical Science, Faculty of Medicine Universitas Airlangga, Surabaya, 60115, Indonesia; Department of Physics, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang, 65145, Indonesia