IN SILICO STUDY REVEALED THE ANTI-VIRAL POTENCY OF Allium cepa BIOACTIVE COMPOUNDS INHIBIT M-PRO SARS-COV-2 PROTEIN

Closed

Ikrimatul Hasnah, Wira Eka Putra, Sustiprijatno, Arief Hidayatullah, Dahlia Normala, Nurrasilfa, Rani Dwi Lestari, Siti Nurazizah, Yusniar Zurroh Asfiniya, Diana Widiastuti, Hendra Susanto, Muhammad Fikri Heikal, Hary Isnanto

2022 Malaysian Journal of Biochemistry and Molecular Biology Vol. 25 Issue 3 Article Cited by 0 Quartile

Abstract

A zoonotic disease called COVID-19 was initially spread from animals to people. SARS-CoV-2, as opposed to SARS, was a pandemic that resulted in about 274,676,729 cases globally. Numerous studies have looked into potential novel treatments for COVID-19 infection. In this study, 16 phytochemicals from Allium cepa L. were examined in silico for their potential to bind to the primary protease of COVID-19 (PDB ID: 6LU7). Lipinski's rules are used to choose the ligands. Protein protease's (Mpro) Cys-145 and His-41 are its active sites. The chosen ligands are examined using molecular docking with PyRx and Vina Wizard and 2D visualization with LigPlot+. Selected ligands' binding energy value will be contrasted with hydroxychloroquine as a control. According to the results, the compounds luteolin (CID: 5280445), isorhamnetin (CID: 5281654), and apigenin (CID: 5280443), which had binding energies of 7.4, 7.2, and -7 kcal/mol, respectively, become potential COVID-19 inhibitors. These substances have higher binding energies than the control, hydroxychloroquine (CID: 3652), which has a lower binding energy (-6 kcal/mol). Due to its important pharmacokinetic features, luteolin demonstrated the best binding efficacy to Mpro, enabling the development of new drugs. © 2022 Malaysian Society for Biochemistry and Molecular Biology. All rights reserved.

Affiliations

Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, East Java, Indonesia; Biotechnology Study Program, Department of Applied Sciences, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, East Java, Indonesia; Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, West Java, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Pakuan, West Java, Indonesia; Tropical Medicine International Program, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, Thailand