Rohman Mohammad Saifur, Sishartami Lintang Widya, Widodo Nashi, Rachmawati Ermin, Lukitasari Mifetika
Context: Coffee and green tea extract combination is expected to reduce macrophage migration. Aims: We investigated the effects of combination of coffee and green tea extracts on GALNT2 gene expression and PSGL-1 protein expression in Ox-LDL-induced RAW 264.7 cells. Materials and methods: RAW 264.7 cells were treated with a combination of coffee and green tea extracts with three different doses for 24 hours (coffee 80 μg/mL + green tea 80 μg/mL; coffee 160 μg/mL + green tea 160 μg/mL; coffee 320 μg/mL + green tea 320 μg/mL), respectively. Then, cells stimulated with 8 μg/mL Ox-LDL for 20 hours. GALNT2 mRNA expression was analyzed by reverse transcription polymerase chain reaction and western blot for PSGL-1 protein expression. Results: GALNT2 gene expression and PSGL-1 protein expression decreased significantly (P < 0.05) with treatment by combination of coffee and green tea extracts in dose-dependent manner. Conclusion: Coffee and green tea extract combination administration significantly reduced GALNT2 gene expression and PSGL-1 protein expression in Ox-LDL-induced RAW 264.7 cells. © 2022 Authors. All rights reserved.
Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Brawijaya, East Java, Malang, Indonesia; Brawijaya Cardiovascular Research Centre, Universitas Brawijaya, East Java, Malang, Indonesia; Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Brawijaya, East Java, Malang, Indonesia; Department of Public Health, Faculty of Sport Science, Universitas Negeri Malang, East Java, Malang, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, East Java, Malang, Indonesia; Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, UIN Maulana Malik Ibrahim Malang, East Java, Malang, Indonesia; School of Nursing, Faculty of Health Sciences, Universitas Brawijaya, East Java, Malang, Indonesia