Indra Kurniawan Saputra, Annisa Elchamida, Helyu Lystika Afininda, Kennis Rozana, Siti Imroatul Maslikah
Textile industry waste containing lead (Pb) and nickel (Ni) metals from synthetic textile dyes can accumulate in the environment. The countermeasures can be done by bioremediation. The potential microbes for bioremediation agents are derived from the Sidoarjo mudflow due to its metals including Pb and Ni. The characterization of remediation microbes in this study aims to provide basic knowledge on their physical and biochemical properties, as well as their level of sensitivity to antibiotics. Microbial sampling was conducted approximately 500 m from the center of the Porong Sidoarjo eruption mud, East Java. Samples were cultured with 0.1 mM PbNi metals nutrient agar. The isolate was selected for further characterization. Morphologically, the colony has smooth edges, flat elevation, and is shiny. Biochemically, the colony is gram positive, oxidase negative, catalase-positive, and indole negative. Meanwhile, antibiotic testing results suggest that all antibiotics are categorized as susceptible, except for tetracycline and kanamycin that are categorized as intermediate for the Neisseria sp. or Staphylococcus spp isolate. The antibiotics with a high susceptible level are ampicillin, amoxicillin, and doxycycline with zones of inhibition 52.23 mm, 49.13 mm, and 47.78 mm, respectively. Accordingly, the three antibiotics are recommended to control the growth of the isolates. © 2023 American Institute of Physics Inc.. All rights reserved.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang, Indonesia; Biotechnology Program Study, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang, Indonesia