I. Gusti Aju Wahju Ardani, Melisa Budipramana, Erlina Rachmawati, Alexander Patera Nugraha, I. Kade Karisma Gita Ardana, Theresia Indah Budhy, Rozita Hassan, Dwi Listyorini, Riyanarto Sarno
Objective The aim of this article is to analyze and compare the presence of single-nucleotide polymorphisms (SNPs) of COL1A1 and FGFR2 in class II and class III Javanese populations. Materials and Methods Cephalometric radiographs from total 63 patients of class II and III were analyzed. SNP analysis was performed based on both COL1A1 and FGFR2 sequences amplified from total DNA of patients' fresh blood. Principal component analysis was done to calculate the data and find the correlation of the cephalometric indicators influenced by each mutation. t -test and Mann-Whitney analysis were performed to check the significance of differences occurred in each studied parameter (p < 0.05). Result There were three COL1A1 SNPs found in class II and only two in class III, while three FGFR2 SNPs found in both classes. Class II phenotype seemed to be strongly influenced by Y-axis and mandibular plane angle, while class III by lower gonial angle and mandibular plane angle. Conclusion Based on this study, we suggest that rs2249492 of COL1A1 and rs2981582 of FGFR2 play important roles in class III, while rs2277632 of COL1A1 and rs2981582 of FGFR2 play important role in class II skeletal malocclusion in Javanese population. © 2023 Georg Thieme Verlag. All rights reserved.
Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia; Department of Orthodontics, Faculty of Dental Medicine, Universitas Lambung Mangkurat, Banjarmasin, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang, Indonesia; Department of Oral and Maxillofacial Pathology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia; Orthodontic Unit, School of Dental Sciences, Universiti Sains Malaysia, Kelantan, Malaysia; Department of Biotechnology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang, Indonesia; Department of Informatics, Institute Technology of Sepuluh Nopember, Surabaya, Indonesia