Diana Widiastuti, Siska Elisahbet Sinaga, Siti Warnasih, Erna Pujiyawati, Supriatno Salam, Wira Eka Putra
Averrhoa bilimbi L., (Belimbing wuluh), is traditionally used for various antidiabetic, anti-larvicidal, antioxidant, hypoallergenic, antibacterial, antifungal, and anti-inflammatory treatments. This study evaluated 3 extracts (n-hexane, ethyl acetate, and n-butanol) isolated from starfruit flowers for antidiabetic effects using in vitro and in silico methods. Ethyl acetate extract showed the highest activity, followed by n-hexane and n-butanol extracts in inhibiting α-glucosidase. Each extract has an IC50 value of 89.84, 52.88, and 114.86 µg/mL. The active compounds contained in the ethyl acetate extract were determined using LC-MS, and the compounds with the highest retention time were identified as tautomycetin (8.585) and lithocholic acid (8.993). Tautomycetin and lithocholic acid showed promising results in the in silico test. The computational predictions showed that tautomycetin (−6.2 kcal/mol) and lithocholic acid (−10.0 kcal/mol) have better binding affinity values than miglitol (−5.4 kcal/mol). This is the first time the antidiabetic test of starfruit flower extract has been carried out. © 2023, Walailak University. All rights reserved.
Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Pakuan, West Java, Indonesia; Department of Nutritional Science, Universitas Widya Nusantara, Palu, Indonesia; Department of Pharmacy, Faculty of Pharmacy, Universitas Mulawarman, Kalimantan, Indonesia; Biotechnology Study Program, Department of Applied Sciences, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, East Java, Indonesia