Indra Kurniawan Saputra, Annisa Elchamida, Helyu Listyka Afininda, Seprianto Seprianto, Sitoresmi Prabaningtyas
The Sidoarjo Mud is one of the natural disasters that spew hot mud containing metal, which occurred in Sidoarjo Regency, East Java, Indonesia. The microbes growing in the area show a potential candidate as a bioremediation agent for chicken farm waste. The main heavy metals in livestock industry waste are Copper (Cu) and zinc (Zn). The microbes were isolated and cultured with Nutrient Agar media by addition 0.1 mM CuZn. Then, profile analysis was carried out in the isolate, including the morphological, biochemical, and antimicrobial. The result showed that only one isolate has successfully grown in 3 times repetition. Morphologically, the isolate's colony is yellow, with irregular form, curved edges, gloomy density and the microscopic of the isolate was coccus. Biochemically, the isolate is Gram-negative, catalase-positive, oxidase-positive, and indole-positive. Moreover, for antimicrobial susceptibility testing, all antibiotics were categorized as susceptible except Tetracycline (TE) and chloramphenicol (C) which belong to intermediate and resistant. The best antibiotic to control the growth of the isolate is doxycycline and kanamycin because of zone diameter is higher than other antibiotics. This study concluded that the isolate has a potential prospect for overcoming Cu and Zn contaminant, which is produced by chicken farm waste. © 2023 American Institute of Physics Inc.. All rights reserved.
Department of Biology, Faculty Mathematics and Natural Sciences, Universitas Negeri Malang, Malang, Indonesia; Biotechnology Study Program, Faculty Mathematics and Natural Sciences, Universitas Negeri Malang, Malang, Indonesia; Study Program of Biotechnology, Faculty of Health, Universitas Esa Unggul, Jakarta, Indonesia
