Raisha Anis Fatiha, Christian Kencana Ngabdi, Jihan Salwa Azizah, Bintang Sukma Natalsah Putri, Bayu Lestari, Nik Ahmad Nizam Nik Malek, Hendra Susanto, Wibi Riawan, Happy Kurnia Permatasari
Objective: This study aimed to evaluate the potential anticancer effect of Moringa oleifera leaf powder–silver nanoparticles (MOLP-AgNPs) by assessing their impact on epithelial–mesenchymal transition (EMT) markers and cell migration in HeLa cervical cancer cells. Methods: HeLa cells were treated with MOLP-AgNPs at concentrations of 2, 4, and 6 µg/mL, alongside an untreated control group. Cell viability was assessed using the trypan blue exclusion method to determine non-toxic concentrations. A scratch (wound-healing) assay was used to evaluate cell migration, while immunofluorescence staining was performed to detect the expression of EMT-related proteins Snail and vimentin. Result: MOLP-AgNPs significantly inhibited cell migration in a dose-dependent manner (p < 0.05). Additionally, the expression of Snail and vimentin was markedly downregulated following treatment, indicating suppression of EMT-related pathways (p < 0.001). Conclusion: These exploratory preclinical findings suggest that MOLP-AgNPs exert anti-migratory effects on cervical cancer cells by modulating EMT. Although HeLa is a non-metastatic cell line, this study highlights the potential of MOLP-AgNPs as a promising candidate for further investigation in metastatic cancer models. © This work is licensed under a Creative Commons Attribution-Non Commercial 4.0 International License.
Faculty of Medicine, Universitas Brawijaya, Malang, 65145, Indonesia; Pharmacology Department, Faculty of Medicine, Universitas Brawijaya, Malang, 65145, Indonesia; Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, Skudai, 81310, Malaysia; Centre for Sustainable Nanomaterials (CSNano), Ibnu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia, Skudai, 81310, Malaysia; Department of Biology, Faculty of Mathematics and Science, Universitas Negeri Malang, Malang, 65145, Indonesia; Department of Biochemistry and Biomolecular, Faculty of Medicine, Universitas Brawijaya, Malang, 65145, Indonesia
