Sri Rahayu Lestari, Abdul Gofur, Dra. Hartatiek, Yuslinda Annisa, Dimas Nur Ramadhani, Amalia Nur Rahma, Dahniar Nur Aisyah, Ikfi Nihayatul Mufidah, Nadiya Dini Rifqi
Background: Single-bulb garlic extract (SBGE) contains more active compounds than regu-lar garlic, but it is unstable and easily degraded in the digestive tract. SBGE is expected to be protected by microencapsulation chitosan-alginate (MCA). Objective: The present study aimed to characterize and assess the antioxidant activity, hemocompatibil-ity, and toxicity of MCA-SBGE in 3T3-L1 cells. Methods: The research procedures consist of extraction of single bulb garlic, preparation of MCA-SBGE, Particle Size Analyzer (PSA), FTIR analysis, DPPH assay, hemocompatibility test, and MTT assay. Results: The average size of MCA-SGBE was 423.7 ± 2.8 nm, the polydispersity index (PdI) was 0.446 ± 0.022, and the zeta potential was-24.5 ± 0.4 mV. MCA-SGBE was spherical with a diameter range of 0.65-0.9 µm. A shift in absorption and addition of functional groups was found in SBGE after encapsu-lation. MCA-SBGE, at a concentration of 24 x 103 ppm, has higher antioxidants than SBGE. The hemo-compatibility test shows the hemolysis of MCA-SBGE lower than SBGE. MCA-SBGE was not toxic to 3T3-L1 cells with cell viability percentage above 100% at all concentrations. Conclusion: MCA-SBGE characterization has microparticle criteria with homogeneous PdI values, low particle stability, and spherical morphology. The results showed that SBGE and MCA-SBGE are non-hemolytic, compatible with red blood cells, and non-toxic to 3T3-L1 cells. © 2024 Bentham Science Publishers.
Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Malang, East Java, Malang, 65145, Indonesia; Department of Physics, Faculty of Mathematics and Natural Science, Universitas Negeri Malang, East Java, Malang, 65145, Indonesia; Department of Biology, Faculty of Mathematics and Natural Science, Universi-tas Brawijaya, East Java, Malang, 65145, Indonesia
