Rizki Handayani, Hendra Susanto, Sri Rahayu Lestari, Nik Ahmad Nizam Nik Malek, Khulud Rizqi Azizah
Liver fibrosis represents a progressive pathological stage leading to cirrhosis and hepatocellular carcinoma, primarily mediated by the TGF- β/SMAD signaling pathway. This study aimed to evaluate the effect of Moringa oleifera Seed Oil (MOSEIL) on TGF-β expression in Mus musculus (Balb/c) with CCl4 -induced liver fibrosis. Experimental groups consisted of a normal control (K0), olive oil control (K-), positive control (K+), and three treatment groups (P1- P3) receiving different ratios of CCl4: MOSEIL, namely P1 (1:8), P2 (1:16), and P3 (1:32).Immunohistochemical analysis revealed a marked increase in TGF-β expression in the positive control group, while MOSEIL administration resulted in a dose-dependent reduction in expression intensity, with the most prominent effect observed in P2. Histological observations supported these findings, showing decreased necrosis and inflammatory cell infiltration in P1 and P2. In contrast, the P3 group demonstrated a re-elevation of inflammatory activity and fibrotic features, suggesting that excessive dosing may attenuate the protective effects of MOSEIL. The reduced expression of TGF-β in MOSEIL-treated groups indicates that MOSEIL exerts its antifibrotic activity partly through inhibition of this profibrotic cytokine. Therefore, MOSEIL exhibits potential as a natural hepatoprotective and anti-fibrotic agent against chronic liver injury, with an optimal effect observed at a moderate dose. © 2026 The Authors, published by EDP Sciences.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Semarang St. 5, Malang, 65145, Indonesia; Departement of Biosciences, Faculty of Sciences, Universiti Teknologi Malaysia, Iman St. Johor Darul Ta-zim, Skudai, 81310, Malaysia
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