A. Pranoto, S. Sugiharto, P.S. Rejeki, A. Hariyanto, G.F. Yosika, B.A. Pramono, I.D.M.A. Wijaya, R.A.A. Nikmah, B. Ristiawan, B.E. Orhan
Obesity is characterised by excessive body fat accumulation, leading to an imbalance in myokine secretion, contributing to increased low-grade chronic inflammation. Therefore, strategies to reduce fat accumulation and control inflammation are essential in obesity management. This study aims to investigate the changes in serum irisin, peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC-1α), and interleukin (IL)-6 induced by combined strength-endurance training in obese young adult women. A total of 24 obese women aged 20-25 years, with a body fat percentage of ≥30%, were recruited and randomly assigned to either a strengthendurance training group (n = 12) or a control group (n = 12). Only the training group underwent an eightweek strength-endurance intervention. Enzyme-linked immunosorbent assay (ELISA) was used to analyse serum concentrations of irisin, PGC-1α, and IL-6 in all samples. Statistical analysis using an mixed-model ANOVA with a significance level of 5% and effect size analysis using Cohen's d. Significant differences were observed between the control and training groups in post-irisin (Effect size (ES): 2.283), Δ-irisin (ES: 2.031), post-PGC-1α (ES: 1.571), Δ-PGC- 1α (ES: 1.155), post-IL-6 (ES: 0.955), and Δ-IL-6 (ES: 1.438) (all P ≤ 0.05). This study found increased serum irisin concentration, elevated expression of PGC-1α, and a decreased concentration of the pro-inflammatory cytokine IL- 6 in young adult women with obesity following eight weeks of strength-endurance training. These findings have significant implications for obesity management through non-pharmacological, exercise-based strategies. © A. Pranoto et al., 2025.
Department of Sports Coaching Education, Faculty of Sports and Health Science, Universitas Negeri Surabaya, Surabaya, Indonesia; Sport and Exercise Research Center, Universitas Negeri Surabaya, Surabaya, Indonesia; Department of Sports Sciences, Faculty of Sports Sciences, Universitas Negeri Malang, Malang, Indonesia; Physiology Division, Department of Medical Physiology and Biochemistry, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; Study Program of Sports Coaching Education, Faculty of Teacher Training and Education, Universitas Tanjungpura, Pontianak, Indonesia; Department of Physical Education Health and Recreation, Faculty of Sports and Health Science, Universitas Negeri Surabaya, Surabaya, Indonesia; Department of SportManagement, Faculty of Sports and Health Science, Universitas Negeri Surabaya, Surabaya, Indonesia; Faculty of Sports Sciences, Istanbul Aydin University, Istanbul, 34295, Turkey
