M.J. Ramadhan, N. Choirunisa, S. Kharomah, M. Agustin, M.T. Hilali, A. Fauzi, D. Setiawan, S. Zubaidah
Bisphenol A (BPA) and acrylamide (ACR) are environmental contaminants of increasing concern due to their widespread presence and potential toxicological effects. This study investigates the impact of BPA and ACR exposure on the feeding behavior of Drosophila melanogaster, using wild-type, white, and black strains as model organisms. A multi-generational design spanning three generations with four replicates per treatment was applied. Feeding preference was assessed through a color-based medium choice assay, where abdominal coloration indicated dietary intake after exposure to red-colored (contaminated) and blue-colored (uncontaminated) media. Experimental groups were exposed to BPA (0.25 and 0.5 mg/mL) and ACR (2.4 and 4.8 mg/kg bw/day). Following a six-hour fasting period, flies consistently showed preference for the contaminated medium. The strongest response was observed in the 2.4 mg/kg bw/day ACR group (51.11%), followed by 0.25 mg/mL BPA (50.14%), 0.5 mg/mL BPA (49.86%), 4.8 mg/kg bw/day ACR (48.75%), and the control (44.58%). A subset of flies exhibited avoidance by not feeding. These findings suggest that sublethal exposure to BPA and ACR alters feeding preferences in D. melanogaster, reinforcing its utility as a model for ecotoxicological studies on behavioral impacts of environmental toxicants. © Published under licence by IOP Publishing Ltd.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Indonesia; Department of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah Malang, Indonesia
