Habib Syaiful Arif Tuska, Anik Martinah Hariati, Anwar Sanusi, Gatot Ciptadi, Barlah Rumhayati, Hendra Susanto, Gretania Residiwati, Aulanni’Am Aulanni’am
Heavy metal pollution in freshwater ecosystems poses critical threats to fish health, particularly reproductive function. The Brantas River, one of Indonesia’s most economically important watersheds, has experienced escalating contamination due to industrial, agricultural, and domestic waste discharges, leading to ecological concern. This study aimed to evaluate the bioaccumulation and toxicological effects of heavy metals on histopathological alterations (necrosis, inflammation, hyperemia, and hyperplasia) in gills and testes, and on sperm quality (viability, DNA integrity, and morphological abnormalities) of male Nile tilapia (Oreochromis niloticus) from the Brantas River Basin, Malang, East Java, Indonesia. Water and tissue samples were collected from five river sites (25 male fish; 5 fish per site). Heavy metal levels in water were analyzed using Atomic Absorption Spectrophotometry (AAS). Histopathological scores were evaluated semi-quantitatively. Sperm viability, DNA fragmentation, and morphology were assessed microscopically. Statistical analyses included One-Way ANOVA and Spearman correlation tests (IBM SPSS 23.0; p<0.05). Cadmium (Cd) and iron (Fe) were found at elevated concentrations, particularly at Kalisari (Cd = 0.0129 ppm; Fe = 7.690 ppm]), exceeding WHO (0.003 and 0.3 ppm) and USEPA (0.005 and 0.3 ppm) guidelines. Fish from this site exhibited the most severe histopathological alterations in both gills and testes: necrosis (1.8±0.24; 2.27±0.70), inflammation (2.13±0.27; 2.00±0.85), hyperemia (1.93±0.27; 2.60±0.63), and hyperplasia (2.07±0.27; 2.33±0.72), respectively. Sperm quality was significantly compromised: viability (60.27±1.17%), DNA fragmentation (59.4±0.87%), and morphological abnormalities (43±1.47%) (p<0.05). Strong positive correlations were found between Fe levels and testicular inflammation (r = 0.909), testicular hyperplasia (r = 0.921), and gill hyperplasia (r = 0.913) (p<0.05). Cd was negatively correlated with sperm plasma membrane integrity (r =-0.927) and DNA integrity (r =-0.896) (p<0.05). In conclusion, chronic exposure to Cd and Fe in the Brantas River is associated with marked tissue pathology and reduced reproductive performance in Nile tilapia. These findings underscore the urgent need for integrated river health monitoring and pollution control strategies to protect aquatic biodiversity and sustain ecosystem services in the Brantas River Basin. © 2025, Society for Indonesian Biodiversity. All rights reserved.
Doctoral Program of Environmental Science, Universitas Brawijaya, Jl. MT Haryono 169, East Java, Malang, 65145, Indonesia; Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Brawijaya, Jl. Puncak Dieng, East Java, Malang, 65151, Indonesia; Faculty of Fisheries and Marine Science, Universitas Brawijaya, Jl. Veteran, East Java, Malang, 65145, Indonesia; Ministry of Manpower of the Republic of Indonesia, Jl. Gatot Subroto No. 51, Jakarta, South Jakarta, 12950, Indonesia; Faculty of Animal Science, Universitas Brawijaya, Jl. Veteran, East Java, Malang, 65145, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Jl. Veteran, East Java, Malang, 65145, Indonesia; Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang. Jl, Semarang 5, Malang 6514, East Java, Indonesia; Department of Veterinary Anatomy, Histology, and Embryology, Faculty of Veterinary Medicine, Universitas Brawijaya, Jl. Puncak Dieng, East Java, Malang, 65151, Indonesia
