Fauzi Akhbar Anugrah, Rahmi Masita, Siti Zubaidah
Endophytic bacteria A13 in the previous study were known to produce metabolites similar to the cinchona plant in liquid media. This research used sugarcane juice as an alternative media by modifying the carbon and nitrogen (C/N) ratio for growth bacteria and evaluated the secondary metabolite production by endophytic bacteria. The results of the analysis of the dry matter of sugarcane juice showed that the total carbon and nitrogen were 1.08% and 0.003%, respectively. Bacteria were grown on a modified liquid media by adding urea so that the C/N ratio of the media became 5:1, 7:1, 9:1, and 11:1. The optimization of the C/N ratio was used the same initial optimum pH 7 and 30oC incubation temperature from the previous study. The optimum growth media for isolate A13 were C/N 9:1 with a specific growth rate (μ) about 0.53 h-1 and a doubling time (dt) 1,31 h. The cell count reached about 3.51 × 108 CFU/ml at the peak of the Log phase. Furthermore, based on the results of the qualitative Thalleioquin test, it was concluded that the bacteria A13 still shows the potential to produce quinine in the modified sugarcane juice media. © 2025 American Institute of Physics Inc.. All rights reserved.
Department of Biology, Faculty Mathematics and Natural Sciences, Universitas Negeri Malang, Malang, Indonesia
