Aisy Hanifah, Siti Imroatul Maslikah, Sri Rahayu Lestari
The acne vulgaris (AV) skin infection caused by Cutibacterium acnes bacteria has been a global medical issue. The current AV treatment using topical or oral antibiotic formulation carries side effects and leads to bacterial resistance. ZnO nanoparticle retains potential as an antibacterial agent. Generally, the nanoparticle is synthesized through chemical and physical methods. However, those approaches generate toxic residue and require a high operational cost. Therefore, this study adopted environmentally friendly green synthesis, using the soursop (Annona muricata) leaves extracts (Annona muricata). The soursop leaf extract contains secondary metabolites, such as phenolic compounds (flavonoid, tannin), alkaloid, and terpenoids, which have the potential to as well as capping agents preventing the nanoparticle agglomeration, which may reduce its functional effectiveness. This study aims to identify the potential and effective concentration of ZnO nanoparticles as an antibacterial agent against Cutibacterium acnes. This experimental study used complete randomized design with four concentrations of 32 mg/mL (P1), 64 mg/mL (P2), 128 mg/mL (P3), and 256 mg/mL (P4). The obtained data were analyzed using one-way ANOVA (p<0.05), followed by the Duncan test (p<0.01). Our findings showed that ZnO nanoparticles had antibacterial activity, with significant differences between treatments (p=0.00). The most effective concentration of ZnO as an antibacterial was P3 (128 mg/mL), resulting in a sensitive inhibition zone diameter category. © 2025 American Institute of Physics Inc.. All rights reserved.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang 5, Malang, 65145, Indonesia
