Potential Antagonism Test of Bacterial Isolates from Several Lakes in East Java as First Step to Obtain Bacterial Consortium that Accelerates Algae Growth

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M. Aldean Yusuf Khafi, Sitoresmi Prabaningtyas, Dita Ayu Eka Saputri

2025 AIP Conference Proceedings Vol. 3186 Issue 1 Conference paper Cited by 0 Quartile

Abstract

Some potential lake bacteria have the potential as antagonists for the first step to obtain Bacterial Consortium that accelerates algae growth. This lake potential bacteria can produce IAA (17), nitrogen (B2), amylolytic (KN), phosphate (28), and vitamin B12 (G2V1). This study aimed to determine the ability of antagonism between potential lake bacteria. This research was conducted by culturing and incubating bacteria on NB media, then testing the potential antagonists between lake bacteria using paper disks. The test results are in the form of measuring the diameter of the clear zone of bacterial growth using a caliper. Based on the results of the antagonist test, bacteria B2 was able to inhibit the growth of G2V1 bacteria with an average clear zone of 1.21 mm, bacteria 17 was able to inhibit the growth of G2V1 and KN bacteria with an average clear zone of 2.5 mm and 2.03 mm, KN bacteria were able to inhibit the growth of 28 and B2 bacteria with an average clear zone of 1.13 mm and 3.71 mm, respectively, and G2V1 bacteria were able to inhibit the growth of B2 bacteria with an average clear zone of 3.5 mm. Thus, that 17 bacteria, B2, KN, and GN bacteria have antagonistic potential against certain bacteria, while 28 bacteria have no potential. Further, research is needed so that the potential antagonism of potential lake bacteria can be accurately identified. © 2025 American Institute of Physics Inc.. All rights reserved.

Affiliations

Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Semarang Street 5, East Java, Malang, 65112, Indonesia