Daffa' Rizal Dzulfaqaar Alauddin, Iin Lailatul Khoirunnisa, Hani Dwi Siswandini, Rifqi Hilman, Diajeng Ravika Adil Bangun Negara, Sholiha Azizatul Zahro, Yunita Rakhmawati
Paraquat dichloride is one of the active ingredients in the Gramoxone herbicide, which farmers use to control weed growth in crops. The widespread use of the herbicide paraquat raises severe concerns about pollution hazards, one of which is health-related. The herbicide paraquat dichloride can potentially cause lung disorders such as pulmonary fibrosis. This study aimed to determine the effect of exposure to paraquat dichloride on changes in behavior and lung histology of male mice (Mus musculus) due to the formation of scar tissue and thickening of the alveolar walls through the inhalation method. This experimental study randomly divided 24 male mice (Mus musculus) into three groups. K1 is the control group, while K2 is the group that was treated with a solution of the herbicide paraquat dichloride of 50 mg/kg BW dissolved in 1 ml of distilled water for 13 days by inhalation method using a nebulizer. K3 is a treatment group that uses a natural liquid bronchodilator with red pomegranate extract. During the study, it was discovered that treated mice had behavioral changes such as being less active, having a decreased appetite, having increased respiratory frequency, and having inconsistent body weight compared to the control group. The histopathological analysis showed that there was thinning of the alveolar walls due to paraquat dichloride. © 2025 American Institute of Physics Inc.. All rights reserved.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang 5, Malang, 65145, Indonesia
