Dita Ayu Eka Saputri, Sitoresmi Prabaningtyas, Dhiyaudin Aridowi
One way to obtain renewable energy sources is by developing microalgae into biofuels. Microalgae Chlorella vulgaris was chosen as one of the ingredients for biofuel production because of its high oil content, between 20-70% of dry weight. Microalgae co-culture technique with bacteria is applied to produce high biomass and lipid from microalgae processing. The provision of nitrogen-fixing bacteria and IAA-producing bacteria and temperature selection of 20°C, 25°C, 30°C and 35°C are expected to optimize the growth of Chlorella vulgaris. This study aimed to determine the effect of temperature differences, the addition of bacteria, and the interaction between the two on the total cell density of Chlorella vulgaris. The treatment was carried out in a modified photobioreactor by adding a hair dryer as a heater, and a portable air conditioner as a cooler, and a temperature controller to maintain temperature stability. The results of observational data on the 11th day showed that the optimum temperature for growth was 30°C, the addition of nitrogen-fixing bacteria and IAA-producing bacteria, as well as the interaction treatment with the addition of bacteria using a temperature of 30°C had a positive effect on the growth of Chlorella vulgaris cells. © 2025 American Institute of Physics Inc.. All rights reserved.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Semarang Street 5, East Java, Malang City, 65112, Indonesia
