Sri Rahayu Lestari, Muhammad Sasmito Djati, Ahmad Rudijanto, Fatchiyah Fatchiyah
Objective: To determine body weight gain, expression of Igf-1 and Igf-1 receptor on obese rat model treated with rambutan peel extract (RPE) as a physiological response. Methods: Normal and obese rat feed with normal and high calorie diet around 12 weeks and continued to treat with ellagic acid, RPE 15, 30 and 60 mg/kg body weight respectively. Physiological responses observed were weight gain and expression of Igf-1 with its receptor. Body weight of rat was weighed once per week. Expression of Igf-1 and igf-1R observed with fluorescence immunohistochemistry. The intensity of Igf-1 and Igf-1R expression was analysis using FSX-BSW software. Results: The lowest weight gain was obtained on obese rat model treated with RPE 30 mg/kg body weight. The expression of Igf-1 and Igf-1R were reduced on obese rat model treated with RPE compared with obese rat model of non treatment (P<0.05). The low expression of Igf-1 and Igf-1R was found on obese rat model treated with ellagic acid and RPE 30 mg/kg body weight. Conclusions: The RPE was effecting to the physiological response on obese rat model. The RPE 30 mg/kg body weight inhibited body weight gain and decreased the expression of Igf-1 and Igf-1R of obese rat model. © 2014 Asian Pacific Tropical Medicine Press.
Biology Department, Faculty of Mathematics and Sciences, Malang State University, Jl. Semarang No. 5, Malang, East Java, 65145, Indonesia; Department of Biology, Faculty of Sciences, University of Brawijaya, Jl. Veteran, Malang, East Java, 65145, Indonesia; Endocrinology Department, Saiful Anwar Hospital, Jl. Jaksa Agung Suprapto, Malang, East Java, 65145, Indonesia
