Sintje Liline, Mohamad Amin, Umie Lestari, Aloysius Duran Corebima
The identification of Laor worms (Polychaeta) found in marine areas of Ambon Island has been done up till now based on phenotypic characters. The research goal is to find out the species ofthose worms andto determine their kinship relationshipbased on 16S rRNA gene sequences. The data were analyzed using sequencing scanner software (ABI) and multiple alignment was performed using CLUSTAL W, the phylogeny tree reconstruction based on nucleotide sequence was conductedusing 5. 03 MEGA program. The results of the research show that those Laor worm phenotype differs in body shape, color, and size. The results of the phylogenic analysis based on the 16S rRNA gene sequences and the results of genetic distance and similarity analysis relatedshow that the worm samples classfied into 5 groups, namely group A (samples A2, A4, H1, H2, L3, and L4) identified asPalola viridis; group B (samples A5, L1, L2, L6, and L9) identified asEunice fucata; group C (samples A7, H4, H5, L5, and L10) identified asEunice; group D (sample A3) identified as Lumbrineris magnidentata; group E (samples H3, A1, A6, L7, and L8) identified as Nereidae. The value of NJ and ML bootstrap was 28-100% and 22-100% respectively. The genetic distance range between 0. 03851 and 0. 22936 with the highest and lowest similarity 96. 14897% and 77. 06362% respectively. The analysis results based on the 16S rRNA gene sequences of those worms were able to complement the identification phenotypically based on the number of antennas. © 2016, Sphinx Knowledge House. All rights reserved.
Faculty of Teaching and Education, Pattimura University, Ambon, Maluku, Indonesia; Department of Biology, Faculty of Mathematic and Natural Science, The State University of Malang, Malang, Indonesia
