Eko Sulasmi, Murni Sari, Sitoresmi Prabaningtyas
Context: Forest area in Tahura R. Soerjo has high moisture level with rainfall 1488 mm3/year, so it provides an optimum condition for ferns growth. Tannin is one of the secondary metabolites that have many benefits; it can be found in various amounts in plant body parts. Aim: The study aims to identify the tannin in three fern genera, namely, Dryopteris, Asplenium, and Davallia that were obtained from Tahura R. Soerjo. Materials and Methods: Both epiphytic (Davallia) and terrestrial (Asplenium and Dryopteris) fern samples were obtained from Taman Hutan Raya Raden Soerjo, Cangar, Batu city in May-August 2015. Powdered fern samples were extracted through maceration method using methanol (1:10). The methanol extract of fern were tested for the tannin presences using phytochemical screening and layer chromatography. Results: Thin-layer chromatography was done to confirm the screening results that indicate the existence of tannin. Phytochemical screening for gallotannin in methanol extracts positive results. All extracts perform a different number of spot and Rf value.Dryopteris and Davallia frond showed reddish-orange spot on the plate, while Dryopteris rhizome,Davallia rhizome,Asplenium frond, and Asplenium rhizome showed greenish brown on the plate.Conclusion: The phytochemical screening including tannin confirmation and thin-layer chromatography showed that all part (frond and rhizome) from Dryopteris,Davallia, and Asplenium contained gallotannin and tannin. © 2018 Wolters Kluwer Medknow Publications. All rights reserved.
Department of Biology, Faculty of Matemathic and Natural Sciences, Malang State University, Jalan Semarang 5, Malang, 65145, Indonesia
