Annisa Sholikhah, Elhah Nailul Khasna, Dahlia, Dwi Listyorini
One of superior cultivar parameter in rice is a physicochemical character, especially starch content. Starch content variance influencing glycemic index (GI) and controlled by Granule Bound Starch Synthase I (GBSSI) gene expression. GBSSI gene polymorphism may effect on its function in regulating amylose synthesis. This research aimed to discover GBSSI gene polymorphism in local rice from Banyuwangi as the first step in plant breeding to obtain rice with a low GI. We elaborated GBSSI gene sequences to analyze the possibility of gene polymorphism in local rice cultivar from Banyuwangi. The result showed there is GBSSI gene polymorphism composed of insertion or deletion (Indel) and Single Nucleotide Polymorphisms (SNP). This polymorphism caused protein structure changes that suggested influencing amylose content of three rice cultivars. The physicochemical test showed that amylose content of Merah Harum (MH) rice classified as high with 63.4 % ± 14.75 %, Wild-type (WT) rice classified as intermediate with 24.48 % ± 10.24 %, and Hitam Melik (HM) rice classified as low with 15.1 % ± 5.07 %. MH rice has a lowest glycemic index based on amylose content. Indel found in intron 3, exon 7 (MH and WT) and 8 (HM). In addition, SNP found in exon 4 dan exon 7 (MH and WT), and 8 (HM). © 2018 Author(s).
Department of Biology, Faculty of Mathematics and Natural Science, State University of Malang, Malang, Indonesia
